Fibroblast cultures are prepared from tissues obtained on products of conception or stillborn fetuses.
In case of excessive maceration, provide tissue from muscle, kidney, heart or lung.
Send the entire gestational sack if possible. If not available, select tissue containing villi. Fetal parts are difficult to identify early in gestation, and most other tissues, including blood clots, membranes and decidua result in growth of maternal cells
Send either type of specimen to the laboratory the same day, by overnight mail, or refrigerate overnight and send the following day. Samples held up more than three days will not grow.
The tissues are cleaned and dissected in the laboratory. Villi are identified and separated from the remaining tissues. The cleaned tissues are then dissociated with collagenase (fetuses), or collagenase and trypsin (villi). Single cell suspensions are grown in cultures, which are ready to be harvested between 4 and 21 days. The time depends on the condition of the specimen at the time of arrival.
Twenty metaphase cells are analyzed and 2 karyotypes are prepared from each routine specimen.
The analysis will detect fetal sex, and any gross abnormalities of the sex chromosomes or autosomes such as trisomies, monosomies, translocations, deletions and duplications. Deletions and rearrangements on the molecular level cannot be seen.
Results are presented according to the international standards for chromosomal nomenclature (ISCN), and a full explanation of the karyotype is provided. Any abnormal or variant results should be followed by genetic counseling.
Molecular and biochemical testing can also be done on fresh or cultured cells. If the cultures fail to grow and fetal tissue is available then Microarray based Comparative Genomic Hybridization (CGH) studies can be performed by the Molecular laboratory at your request.